Development of an Efficient Cell Disruption Method for Release of Lipoic Acid From Saccharomyces cerevisiae
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چکیده
Production of lipoic acid (LA) is undergoing a paradigm shift with significant preference for fermentative production over conventional chemical synthesis. However, low product yield triggers the importance of the development of an efficient cell disruption method as a critical step in its recovery. In this paper, we report screening of various physical, mechanical and chemical methods of cell disruption for high release of LA from S. cerevisiae. Among these methods, ultra sonication and EDTA were found to be most effective. The optimized ultra sonication process with respect to cell density (10g/L), time of sonication (3 min), acoustic power (50 W) and duty cycle (70%) resulted in maximum release of 11.4 mg/g DCW of LA in one cycle. Although batch release of LA gave a maximum yield of 42.5 mg/g DCW in six cycles of ultra sonication, the high energy consumption during ultra sonication (5400 kJ/ m ) was discouraging during scale-up. Hence, incubation 3 of the cell mass with 50 mM EDTA for 2 h which released 25.59 mg/g DCW of LA was preferred as the method of choice for subsequent work. Assuming LA release during ultra sonication and EDTA permeabilization to follow first order kinetics, the rate constants obtained were 0.011 /min and 0.074 min respectively.
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تاریخ انتشار 2013